Ex-vivo Organ Perfusion: Application to the study of skeletal muscle in rats
In the organ culture system (organ bioreactor), different organs can be perfused by selecting different parts of the organ chambers.
For example, it is possible to purchase one main controller of bioreactor as a shared device for a lab and purchase an individual/exclusively designed chamber unit for one’s specific samples.
In this article, we introduce a case study of a successful perfusion culture of rat skeletal muscle for 7 days.
In the field of skeletal muscle research, applying the Ex-vivo perfusion system will support to achieve better result by filling the gap between animal experiments and cell experiments.
The femoral skeletal muscles were harvested from the rats and cultured in the organ culture system, and after 7 days of perfusion, high perfusion rates were maintained.
Background and aim
To enable an analysis of skeletal muscle isolated from individual animals, we aimed to maintain the activity of the skeletal muscle in a perfusion culture under an analyzable condition, which cannot be analyzed in individual animals.
We aimed to maintain the activity of the skeletal muscle in a perfusion culture under an analyzable condition to enable an analysis of skeletal muscle isolated from individual animals, which cannot be analyzed in individual animals.
The perfusion rate was defined by the following equation
Perfusion rate = return volume from vein ( g ) / pumped volume ( g )
We successfully perfused the cells for 5 days, maintaining a perfusion rate of around 20% (Fig .1).
This allowed for perfusion culture with return fluid from the vein to be analyzed.
As a result of this experiment, perfusion culture was achieved that makes return fluid from the vein to be analyzable.
Sano, K. et al. J Artif Organs (2019) link
Realizing ex-vivo isolated perfusion culture of organs.
Proven results in rat skeletal muscle and rat small intestine
Other organs are also available for customization as much as possible.